SYTOX™ Green Dead Cell Stain is a bright, easy-to-use nucleic acid stain for distinguishing dead from live cells in flow cytometry assays. It is excitable at 488 nm with an emission profile similar to FITC, and has been tested in an assortment of cell types.
Is Sytox green fixable?
Hope this helps. If you want a dead cell stain that you can fix, you will need to search for a fixable dead cell stain (that you apply when most cells are alive, wash out, and then fix). SYTOX Green is not one of these, although some derivative that has a fixable functional group might exist.
What does Sytox green stain?
SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells but penetrates the compromised membranes characteristic of dead cells, making it a useful indicator of dead cells within a population.
How does SYTOX work?
SYTOX Green stain is a high-affinity nucleic acid stain that does not cross the membranes of live cells and yet easily penetrates cells with compromised plasma membranes. This dead-cell indicator was recently used for rapid viability assessment after exposure of cells to disinfectant (7) or antibiotics (16).
How does propidium iodide staining work?
Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between the bases with little or no sequence preference. … PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining.
How do you dilute Sytox greens?
Protocol
- Wash the cells 1–3 times in a phosphate-free buffer as needed.
- Prepare the SYTOX® Green staining solution by diluting the stock solution 1:30,000 (167 nM) in a phosphate-free buffer.
- Add sufficient staining solution to cover the cells.
- Incubate for 15–30 minutes, protected from light.
What does MitoTracker stain?
MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.
How does Sytox blue work?
SYTOX™ Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. … After brief incubation with SYTOX™ Blue stain, the nucleic acids of dead cells fluoresce bright blue when excited with 405 nm violet laser light.