What is Kluver Barrera stain?
Myelin. This combination stain is also called the Kluver-Barrera method and is commonly used to stain nervous tissue. The stain consists of cresyl violet, which stains basophilic structures similarly to hematoxylin, and luxol fast blue which stains the myelin sheath.
What is the myelin stain?
Luxol fast blue is a copper phthalocyanine dye that is soluble in alcohol and is attracted to bases found in the lipoproteins of the myelin sheath. Under the stain, myelin fibers appear blue, neuropil appears pink, and nerve cells appear purple.
What does Luxol fast blue stain?
Product overview. Luxol Fast Blue Stain Kit (Myelin Stain) ab150675 is designed for staining myelin/myelinated axons and Nissl bodies on formalin fixed, paraffin-embedded tissue as well as frozen tissue. This product is used for identifying the basic neuronal structure in brain or spinal cord sections.
What is the fixative used when using Luxol fast blue PAS hematoxylin stain for myelin?
Description: This is for staining of myelin/myelinated axons on formalin-fixed, paraffin-embedded brain and spinal cord tissue sections, as well as frozen sections. Fixation: 10% formalin. … Section: paraffin sections at 5-10 um.
Why is H and E staining used?
Hematoxylin and Eosin (H&E) staining is used routinely in histopathology laboratories as it provides the pathologist/researcher a very detailed view of the tissue. It achieves this by clearly staining cell structures including the cytoplasm, nucleus, and organelles and extra-cellular components.
How does the Nissl stain work?
The Nissl substance (rough endoplasmic reticulum) appears dark blue due to the staining of ribosomal RNA, giving the cytoplasm a mottled appearance. … Individual granules of extra-nuclear RNA are named Nissl granules (ribosomes). DNA present in the nucleus stains a similar color.
What does osmium tetroxide stain?
Osmium Tetroxide is traditionally used in electron microscopy both as a fixative and a heavy metal stain. Osmium Tetroxide is a good fixative and excellent stain for lipids in membranous structures and vesicles. The most prominent staining in adherent human cells (HeLa) is seen on lipid droplets.